New paper was published by our group members Erik van Buijtenen and Jessie van Buggenum together with collaborators Wout Janssen, Paul Vink, Maurice J.M. Habraken, Laura J.A. Wingens, Andrea van Elsas and Hans van Eenennaam! Here we use integrated multi-omic single-cell sequencing technologies to detect and quantify immunoglobulin subclass-specific surface markers, transcriptional profiles and signaling transduction pathway components. We tested whether human antibody secreting cells (ASCs) that only seem to differ in its ability to secrete different IgM, IgA, or IgG antibodies, exhibit other differences that characterize these different ASCs. Our approach detected differential expression of plasmablast and plasma cell markers, homing receptors and TNF receptors. In addition, differential sensitivity was observed for the different cytokine stimulations that were applied during in vitro differentiation. Taken together, our integrated multi-omics approach allowed high-resolution phenotypic characterization of single cells in a heterogenous sample of in vitro differentiated human ASCs.

Congratulations to Erik, Jessie and Wilhelm!!

Click here to read the paper

Nikita, Mathieu, Lía and Wilhelm together with Max Derks from Laboratory of Spectroscopy and Catalysis and Labmate published a new paper on enzyme-based computation! They used proteolytic enzymatic networks immobilised in hydrogel beads and compartmentalised in a CSTR as a novel platform for molecular information processing. They demonstrated robustness of the platform in performing the fundamental arithmetic operations of addition, subtraction and multiplication. This analogue logic approach goes beyond the Boolean gates and has potential applications in future bioelectronic devices. The concept of analogue logic helps to generalize our understanding of computation in catalytic networks.Congratulations!!

You can find the paper here.

New article on photobiocatalysis in flow was published in ACS Biocatalysis in a collaboration with the group of Wolfgang Kroutil from Graz University!

In this paper immobilised photodecarboxylase from C. variabilis (CvFAP) is taken as an example to demonstrate the methodology development of photobiocatalysis in flow conditions. Various enzyme immobilisation techniques were used to determine the best carrier and conversion.

Congratulations to Stefan, Christoph and Wolfgang from Graz University as well as Miglė and Wilhelm!

You can read the paper here.